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Mouse IgA (alpha chain specific) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/5,000-1/20,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Secondary Antibodies
View as table DownloadMouse IgG (Fc specific) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/20,000-1/100,000 Western blot: 1/2,000-1/10,000 Immunohistochemistry: 1/1,000-1/5,000. Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG3 (subclass specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1.000 - 1/5,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/5000-1/20000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100- 1/2500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG (H&L) Antibody Biotin Conjugated
| Applications | WB: 1:2,000 - 1:20,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:350,000 |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:200,000 - 1:300,000 |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:300,000 |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgE (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG (H+L chain) sheep polyclonal antibody, Biotin
| Applications | Suiatble for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/1,000-1/2,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
USD 1,410.00
2 Weeks
Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, Biotin, Purified
| Applications | Immunoassays. ELISA: good binding on plastic Immunohistochemistry on paraffin sections. Indirect Immunofluorescence on human peropheral blood cells. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: For histochemical and Cytochemical use are usually between 1/100 and 1/250 In ELISA and comparable non-precipitating antibody-binding assays between 1/1500 and 1/6000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
USD 455.00
3 Days
Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Biotin
| Applications | Flow Cytometry: < / = 1 µg/10e6 cells. Identification and enumeration of IgE+ cells. Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells . ELISA: 1/5000-1/10000. Enzyme-Linked-Immunosorbent-Assay. |
| Reactivities | Mouse |
| Conjugation | Biotin |
USD 410.00
3 Days
Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Biotin
| Applications | Flow Cytometry: < / = 1 µg/10e6 cells. Identification and enumeration of IgE+ cells. Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells . ELISA. Also used as a second step reagent for mouse IgM monoclonal antibodies. |
| Reactivities | Mouse |
| Conjugation | Biotin |
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