Secondary Antibodies

View as table Download

Equine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/1,000-1/10,000), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/200,000 of the reconstitution concentration is suggested for this product.
Reactivities Horse
Conjugation HRP
Immunogen Horse IgG F(c) fragment.

Equine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot: use at 1/1,000-1/5,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (use at 1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Reactivities Horse
Conjugation HRP
Immunogen Horse IgG F(ab')2 fragment.

Equine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western blot: 1/5,000-1/20,000 or Dot blot), ELISA (1/50,000-1/200,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Horse
Conjugation HRP
Immunogen Horse IgG whole molecule.

Equine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Immunofluorescence: 1/500 - 1/2,500.
Flow Cytometry: 1/2,000 - 1/10,000.
Reactivities Horse
Conjugation HRP
Immunogen Horse IgG F(ab’)2 fragment