Secondary Antibodies

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Sheep IgG F(c) specific (adsorbed) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation HRP

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, HRP

Applications Suitable for Western Blot (1:1,000- 1:10,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (1:500- 1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 m g of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Sheep
Conjugation HRP

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 is suggested for this product.
Reactivities Sheep
Conjugation HRP

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Swine IgG F(ab')2 in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivities Pig
Conjugation HRP

Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:20,000-1:100,000.
Western Blot: 1:2,000-1:10,000.
Reactivities Goat
Conjugation HRP

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/300,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Human
Conjugation HRP

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/10,000-1:100,000.
Westrn Blot: 1/2,000-1/20,000.
Immunohistochemistry: 1:500-1:2,500.
Reactivities Mouse
Conjugation HRP

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immonoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremly low background levels, absence of F(c) mediated binding, lot-to-lot consitency, high titer and specificy.
ELISA: 1:82,000.
Western blot: 1:1,000 - 1:5,000.
Immunohistochemistry: 1:500 - 1:2,500.
Reactivities Rabbit
Conjugation HRP

Rat IgG (H+L chain), Fab Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:10,000 - 1:40,000.
Western Blot: 1:1,000 - 1:4,000.
Reactivities Rat
Conjugation HRP

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product.
Reactivities Goat
Conjugation HRP

Guinea pig IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:40,000 is suggested for this product.
Reactivities Guinea Pig
Conjugation HRP

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (western or dot blot), ELISA, Immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:4,000 - 1:16,000.
Western Blot: 1:500 - 1:2,000.
Reactivities Hamster
Conjugation HRP

Human IgG (adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation HRP

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA 1:10,000 - 1:40,000
Western blot 1:1,000 - 1:5,000
Immunohistochemistry 1:500 - 1:2,500
Reactivities Human
Conjugation HRP

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunhistochemistry: 1/1,000-1/5,000.
Reactivities Human
Conjugation HRP