Secondary Antibodies

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Bovine IgG1 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP

Bovine IgG1 sheep polyclonal antibody, Aff - Purified

Applications ELISA.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG2 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP

Bovine IgG2 (subclass specific) sheep polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG2 in Bovine serum and other body fluids or to determine its concentration.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal goat serum in agar block titration.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG sheep polyclonal antibody, AP

Applications

ELISA: 1/5000-1/50000.
Western Blot: 1/5000-1/50000.

Reactivities Bovine
Conjugation AP

Bovine IgG sheep polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2,000.
Reactivities Bovine
Conjugation FITC

Bovine IgG sheep polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000. 
Western Blot.
 
Reactivities Bovine
Conjugation HRP

Bovine IgG sheep polyclonal antibody, Purified

Applications ELISA: 1/100-1/10,000.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgM sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/5000-1/50000.
Reactivities Bovine
Conjugation AP

Bovine IgM sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on frozen sections: 1/200-1/2000.
Reactivities Bovine
Conjugation FITC

Bovine IgM sheep polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgG1 sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20 - 1/100.
Immunohistochemistry on frozen sections: 1/20 - 1/100.
Reactivities Bovine
Conjugation FITC

Bovine IgG2 sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20 - 1/100.
Immunohistochemistry on frozen sections: 1/20 - 1/100.
Reactivities Bovine
Conjugation FITC

Bovine IgG2 sheep polyclonal antibody, Aff - Purified

Applications ELISA: 1/100 - 1/10000.
Immunodiffusion.
Reactivities Bovine
Conjugation Unconjugated

Bovine IgM sheep polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Bovine
Conjugation HRP