ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal TurboFP635 tag.
TurboFP635 (scientific name Katushka) is a far-red mutant of the red fluorescent protein from sea anemone Entacmaea quadricolor. Possessing excitation/emission maxima at 588/635 nm, TurboFP635 is 7 to 10-fold brighter compared to the spectrally close or mPlum. TurboFP635 is characterized by fast maturation and a high pH-stability and photostability. The unique characteristics of TurboFP635 make it the protein of choice for visualization within living tissues and dual-color high-throughput assays. TurboFP635 is mainly intended for applications where fast appearance of far-red fluorescence is crucial. It is specially recommended for whole body imaging, cell and organelle labeling, and for tracking the promoter activity in auto-fluorescent tissues.
If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal FP635-tagging vector, PS100038.
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