BECN1 Human shRNA Plasmid Kit (Locus ID 8678)
BECN1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector
|Product Name||BECN1 Human shRNA Plasmid Kit (Locus ID 8678)|
|Synonyms||ATG6; beclin1; VPS30|
|Kit Components||BECN1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 8678). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.|
|RefSeq||NM_003766, XM_005257759, XM_005257760, XM_011525421, XM_017025262, XM_017025263, XM_017025264|
|Summary||This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
Beclin-1-p53 interaction is crucial for cell fate determination in embryonal carcinoma cells
,Tripathi, R;Ash, D;Shaha, C;,
J. Cell. Mol. Med. Sep 2014
,PubMed ID 25208472