SaCas9 Staphylococcus aureus Recombinant Protein

CAT#: TP790183

Purified recombinant protein of Staphylococcus aureus SaCas9 containing Simian Virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- terminal of the protein, with N-terminal His tag, expressed in E. coli

Product Images

Purified recombinant protein SaCas9 was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications

Product Data

• Species: Staphylococcus aureus
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MHHHHHHENL YFQGAIAPKK KRKVEDPKKK RKVDMGKRNY ILGLDIGITS
  VGYGIIDYET RDVIDAGVRL FKEANVENNE GRRSKRGARR LKRRRRHRIQ
  RVKKLLFDYN LLTDHSELSG INPYEARVKG LSQKLSEEEF SAALLHLAKR
  RGVHNVNEVE EDTGNELSTK EQISRNSKAL EEKYVAELQL ERLKKDGEVR
  GSINRFKTSD YVKEAKQLLK VQKAYHQLDQ SFIDTYIDLL ETRRTYYEGP
  GEGSPFGWKD IKEWYEMLMG HCTYFPEELR SVKYAYNADL YNALNDLNNL
  VITRDENEKL EYYEKFQIIE NVFKQKKKPT LKQIAKEILV NEEDIKGYRV
  TSTGKPEFTN LKVYHDIKDI TARKEIIENA ELLDQIAKIL TIYQSSEDIQ
  EELTNLNSEL TQEEIEQISN LKGYTGTHNL SLKAINLILD ELWHTNDNQI
  AIFNRLKLVP KKVDLSQQKE IPTTLVDDFI LSPVVKRSFI QSIKVINAII
  KKYGLPNDII IELAREKNSK DAQKMINEMQ KRNRQTNERI EEIIRTTGKE
  NAKYLIEKIK LHDMQEGKCL YSLEAIPLED LLNNPFNYEV DHIIPRSVSF
  DNSFNNKVLV KQEENSKKGN RTPFQYLSSS DSKISYETFK KHILNLAKGK
  GRISKTKKEY LLEERDINRF SVQKDFINRN LVDTRYATRG LMNLLRSYFR
  VNNLDVKVKS INGGFTSFLR RKWKFKKERN KGYKHHAEDA LIIANADFIF
  KEWKKLDKAK KVMENQMFEE KQAESMPEIE TEQEYKEIFI TPHQIKHIKD
  FKDYKYSHRV DKKPNRELIN DTLYSTRKDD KGNTLIVNNL NGLYDKDNDK
  LKKLINKSPE KLLMYHHDPQ TYQKLKLIME QYGDEKNPLY KYYEETGNYL
  TKYSKKDNGP VIKKIKYYGN KLNAHLDITD DYPNSRNKVV KLSLKPYRFD
  VYLDNGVYKF VTVKNLDVIK KENYYEVNSK CYEEAKKLKK ISNQAEFIAS
  FYNNDLIKIN GELYRVIGVN NDLLNRIEVN MIDITYREYL ENMNDKRPPR
  IIKTIASKTQ SIKKYSTDIL GNLYEVKSKK HPQIIKKGGS PKKKRKVEDP
  KKKRKVDTR
• Tag: N-His
• Predicted MW: 130.3 kDa
• Concentration: 5uM
• Purity: > 90% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 10 mM Tris-HCl, pH 7.4, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol
• Endotoxin: < 0.5 EU per 1 µg of the protein by the LAL
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -20℃.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.