SaCas9 Staphylococcus aureus Recombinant Protein

CAT#: TP790183

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Purified recombinant protein of S. pyogenes SaCas9 containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- terminal of the protein, with N-terminal HIS tag, expressed in E. coli


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USD 125.00


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    • 500 pmol


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Product Data
Description Purified recombinant protein of S. pyogenes SaCas9 containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- terminal of the protein, with N-terminal HIS tag, expressed in E. coli
Species Staphylococcus aureus
Expression Host E. coli
Expression cDNA Clone or AA Sequence MHHHHHHENL YFQGAIAPKK KRKVEDPKKK RKVDMGKRNY ILGLDIGITS
VGYGIIDYET RDVIDAGVRL FKEANVENNE GRRSKRGARR LKRRRRHRIQ
RVKKLLFDYN LLTDHSELSG INPYEARVKG LSQKLSEEEF SAALLHLAKR
RGVHNVNEVE EDTGNELSTK EQISRNSKAL EEKYVAELQL ERLKKDGEVR
GSINRFKTSD YVKEAKQLLK VQKAYHQLDQ SFIDTYIDLL ETRRTYYEGP
GEGSPFGWKD IKEWYEMLMG HCTYFPEELR SVKYAYNADL YNALNDLNNL
VITRDENEKL EYYEKFQIIE NVFKQKKKPT LKQIAKEILV NEEDIKGYRV
TSTGKPEFTN LKVYHDIKDI TARKEIIENA ELLDQIAKIL TIYQSSEDIQ
EELTNLNSEL TQEEIEQISN LKGYTGTHNL SLKAINLILD ELWHTNDNQI
AIFNRLKLVP KKVDLSQQKE IPTTLVDDFI LSPVVKRSFI QSIKVINAII
KKYGLPNDII IELAREKNSK DAQKMINEMQ KRNRQTNERI EEIIRTTGKE
NAKYLIEKIK LHDMQEGKCL YSLEAIPLED LLNNPFNYEV DHIIPRSVSF
DNSFNNKVLV KQEENSKKGN RTPFQYLSSS DSKISYETFK KHILNLAKGK
GRISKTKKEY LLEERDINRF SVQKDFINRN LVDTRYATRG LMNLLRSYFR
VNNLDVKVKS INGGFTSFLR RKWKFKKERN KGYKHHAEDA LIIANADFIF
KEWKKLDKAK KVMENQMFEE KQAESMPEIE TEQEYKEIFI TPHQIKHIKD
FKDYKYSHRV DKKPNRELIN DTLYSTRKDD KGNTLIVNNL NGLYDKDNDK
LKKLINKSPE KLLMYHHDPQ TYQKLKLIME QYGDEKNPLY KYYEETGNYL
TKYSKKDNGP VIKKIKYYGN KLNAHLDITD DYPNSRNKVV KLSLKPYRFD
VYLDNGVYKF VTVKNLDVIK KENYYEVNSK CYEEAKKLKK ISNQAEFIAS
FYNNDLIKIN GELYRVIGVN NDLLNRIEVN MIDITYREYL ENMNDKRPPR
IIKTIASKTQ SIKKYSTDIL GNLYEVKSKK HPQIIKKGGS PKKKRKVEDP
KKKRKVDTR
Tag N-His
Predicted MW 130.3 kDa
Concentration 5uM
Purity > 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer 10mM Tris,300mM NaCl,0.1mM EDTA,1mM DTT, pH7.4, 50% glycerol.
Endotoxin < 0.5 EU per 1 µg of the protein by the LAL
Storage Store at -20℃ after receiving vials.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
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