TEV protease Tobacco etch virus Recombinant Protein

CAT#: TP790024

Purified recombinant protein of TEV protease, with N-terminal HIS tag, expressed in E.Coli

Product Images

Purified recombinant protein TEV protease was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications

Product Data

• Species: Tobacco etch virus
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MHHHHHHMGE SLFKGPRDYN PISSTICHLT NESDGHTTSL YGIGFGPFII TNKHLFRRNN
  GTLLVQSLHG VFKVKNTTTL QQHLIDGRDM IIIRMPKDFP PFPQKLKFRE PQREERICLV
  TTNFQTKSMS SMVSDTSCTF PSSDGIFWKH WIQTKDGQCG SPLVSTRDGF IVGIHSASNF
  TNTNNYFTSV PKNFMELLTN QEAQQWVSGW RLNADSVLWG GHKVFMVKPE EPFQPVKEAT
  QLMN
• Tag: N-HIS
• Predicted MW: 27.8KDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 90% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 5 mM DTT, 50% glycerol
• Bioactivity: 1 unit is defined as the amount of enzyme required to cleave 3 µg of a substrate protein to 85% completion in 1 hours at 30°C.
• Endotoxin: < 1 EU per 1 µg of the protein by the LAL
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -20°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.