Histone H2A.X (H2AFX) (NM_002105) Human Recombinant Protein

CAT#: TP761594

Purified recombinant protein of Human H2A histone family, member X (H2AFX), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding human full-length H2AFX
• Tag: N-GST and C-His
• Predicted MW: 43 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_002096
• Locus ID: 3014
• UniProt ID: P16104
• Cytogenetics: 11q23.3
• Refseq Size: 1651
• Refseq ORF: 429
• Synonyms: H2A.X; H2A/X; H2AFX
• Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]
• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus