Tn5 Escherichia coli Recombinant Protein

CAT#: TP750177

Purified recombinant protein of Tn5 transposase, with N-terminal protein A tag, expressed in E.Coli, 50 ug

Product Images

Purified recombinant protein Tn5 was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications

Product Data

• Species: Escherichia coli
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGDYKDHDGD YKDHDIDYKD DDDKMTMITP SLKDDPSQSA NLLSEAKKLN ESQAPKADNK
  FNKEQQNAFY EILHLPNLNE EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNKFN
  KEQQNAFYEI LHLPNLTEEQ RNGFIQSLKD DPSVSKEILA EAKKLNDAQA PKDDDKEFGG
  GGSGGGGSGG GGSGGGGSHM ITSALHRAAD WAKSVFSSAA LGDPRRTARL VNVAAQLAKY
  SGKSITISSE GSKAMQEGAY RFIRNPNVSA EAIRKAGAMQ TVKLAQEFPE LLAIEDTTSL
  SYRHQVAEEL GKLGSIQDKS RGWWVHSVLL LEATTFRTVG LLHQEWWMRP DDPADADEKE
  SGKWLAAAAT SRLRMGSMMS NVIAVCDREA DIHAYLQDKL AHNERFVVRS KHPRKDVESG
  LYLYDHLKNQ PELGGYQISI PQKGVVDKRG KRKNRPARKA SLSLRSGRIT LKQGNITLNA
  VLAEEINPPK GETPLKWLLL TSEPVESLAQ ALRVIDIYTH RWRIEEFHKA WKTGAGAERQ
  RMEEPDNLER MVSILSFVAV RLLQLRESFT PPQALRAQGL LKEAEHVESQ SAETVLTPDE
  CQLLGYLDKG KRKRKEKAGS LQWAYMAIAR LGGFMDSKRT GIASWGALWE GWEALQSKLD
  GFLAAKDLMA QGIKICITGD ALVALPEGES VRIADIVPGA RPNSDNAIDL KVLDRHGNPV
  LADRLFHSGE HPVYTVRTVE GLRVTGTANH PLLCLVDVAG VPTLLWKLID EIKPGDYAVI
  QRSAFSVDCA GFARGKPEFA PTTYTVGVPG LVRFLEAHHR DPDAQAIADE LTDGRFYYAK
  VASVTDAGVQ PVYSLRVDTA DHAFITNGFV SHATGLTG
• Tag: N-PROTEIN
• Predicted MW: 75KDa
• Concentration: >0.05 µg/µL as determined by Bradford protein Assay method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50 mM HEPES-KOH, pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -20°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.