Grancalcin (GCA) (NM_012198) Human Recombinant Protein
CAT#: TP720123M
Recombinant protein of human grancalcin, EF-hand calcium binding protein (GCA)
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Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
Met1-Ile217
|
Tag | N-GST |
Predicted MW | 50.2 kDa |
Concentration | lot specific |
Purity | >95% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | Provided lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl |
Endotoxin | < 0.1 EU per µg protein as determined by LAL test |
Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Dissolve the lyophilized protein in ddH2O. It is not recommended to reconstitute a concentration less than 100 µg/ml. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Storage | Store at -80°C. |
Stability | Stable for at least 6 months from date of receipt under proper storage and handling conditions. |
Reference Data | |
RefSeq | NP_036330 |
Locus ID | 25801 |
UniProt ID | P28676 |
Cytogenetics | 2q24.2 |
Synonyms | GCL |
Summary | This gene encodes a calcium-binding protein that is abundant in neutrophils and macrophages. In the absence of divalent cation, this protein localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions. Alternative splicing and use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Aug 2016] |
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