Ttll11 (NM_029774) Mouse Recombinant Protein

CAT#: TP525693

Purified recombinant protein of Mouse tubulin tyrosine ligase-like family, member 11 (Ttll11), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR225693 representing NM_029774
  Red=Cloning site Green=Tags(s)
  
  MRRSSPEKKPEAEWEADAAAAAAATAAATESLPAETEKQQGVDAGAAGDPERLELEEQPKDVGRIPTPTR
  RHAPEEGEARVVRRLPPALPLAQPRPAARALSQLVKARGRSRSRVYRRSAGSMRPVTVDSSKARTSLDAL
  KISLRQLRWKEFPFGRRLPCDIYWHGVSFRDSDILSGQVNKFPGMTEMVRKVTLSRALRIMQNLFPEEYN
  FYPRSWILPEEFQLFVSQVQTVKEGDPSWKPTFIVKPDSGCQGDGIYLIKDPCDGRLTGTLHNRPAVVQE
  YIRKPLLIDKLKFDIRLYVLLKSLDPLEIYIAKDGLSRFCTEPYQEPNPQNLHHVFMHLTNYSLNIHSGK
  FVHSDSASTGSKRTFSSILCRLSSKGVDIKKVWSDIISLVIKTVIALTPELKVFYQSDIPTGRPGPTCFQ
  ILGFDILLMKNLKPMLLEVNANPSMRIEHEYELSPGVFENIPSLVDEEVKVAVIRDTLRLMDPLKKKKEI
  HFPDIYMDRKHRIPPVSDRMSSWKHKGSSLSIVRSQQMEKSFTSKEDLNCDPTGGDSEPNPEAHLPSICL
  KQVFPKYAKQFNYLRLVDRMANLFIRFLGIKGTMKLGPTGFRTFIRNCKLSSSSLSMAAVDILYIDITRR
  WNSVTVDQRDSGMCLQAFVEAFFFLAQRKFKLQPLHEQVASLIDLCEYHLSVLDEKRLLCHRGRPLQRNP
  PQMNRPEHSATGSSAPRVIGASKLSQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 82.4 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_084050
• Locus ID: 74410
• UniProt ID: A4Q9F4
• Cytogenetics: 2 23.9 cM
• Refseq Size: 3191
• Refseq ORF: 2181
• Synonyms: 4932702F08Rik; 4933424A20Rik; AU043211; D2Ertd624e
• Summary: Polyglutamase which preferentially modifies alpha-tubulin (PubMed:17499049). Involved in the side-chain elongation step of the polyglutamylation reaction rather than in the initiation step. Required for CCSAP localization to both spindle and cilia microtubules (PubMed:17499049). Generates long side-chains (PubMed:20530212).[UniProtKB/Swiss-Prot Function]