Nek11 (NM_172461) Mouse Recombinant Protein

CAT#: TP518193

Purified recombinant protein of Mouse NIMA (never in mitosis gene a)-related expressed kinase 11 (Nek11), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR218193 representing NM_172461
  Red=Cloning site Green=Tags(s)
  
  MLKFQETAKCVGRRPTVIPMYPTALIARRYVLQQKLGSGSFGTVYLVSDKKAKPGEELKVLKEISVGELN
  PNETVQANVEAQLLSRLHHPAIVRFHASFMEQETFCIITEYCEGRDLDYRIQEYKEAGKVFAENQIVEWF
  IQLLLGVDYMHERRILHRDLKSKNIFLKNNLLKIGDFGVSRLLMGSCELATTLTGTPHYMSPEALKHQGY
  DAKSDIWSLACILYEMCCLDHAFAGSSFLSVVLNIVEGKTPSLPDRYPRELNTIMERMLNKSPSLRPSAA
  DILKAPYMEEQLQLLMCKYPEMTLEDKNSVCQKEAAHTINAVQKKLHLQTLQALSDTQKTTPRERMWLRK
  LQAADERARRLKKIAEENYKENDKRMQALRSRNVGSVHAHVLHELDERTLESLPEPQSLPCLDLDELEPS
  LEDTIVDLGHYEIPEDPLVAEQYYSDVFDSCSEDSEEQEEEMIFSEAGGDTKEEESPSVYRTNQQDSDTA
  ALVGCLEHVLGYTSLDTKTITNAVTDMSPGPMVFNSAVARTKMKRMKESAVQKLGMETFEEVYDYLKRAR
  HQNAREAEIWEHLETVVPRASDCFEVDQLLYFEELLLTMEGKEPSLQNLPCEAAQKKPVKGTHFCDNP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 72.1 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_766049
• Locus ID: 208583
• UniProt ID: Q8C0Q4
• Cytogenetics: 9 F1
• Refseq Size: 2625
• Refseq ORF: 1884
• Synonyms: 4932416N14Rik
• Summary: Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.[UniProtKB/Swiss-Prot Function]