P3h1 (NM_001042411) Mouse Recombinant Protein

CAT#: TP510385

Purified recombinant protein of Mouse prolyl 3-hydroxylase 1 (P3h1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR210385 representing NM_001042411
  Red=Cloning site Green=Tags(s)
  
  MAVSERRLLAAMLAVAAAAALRVAAESEPGWDVAAPDLLYAEGTAAYSRGDWPGVVLNMERALRSRAALR
  ALRLRCRTRCATELPWAPDLDLXXPDPSLSQDPGAAALHDLRFFGAVLRRAACLRRCLGPPSAHLLSEEL
  DLEFNKRSPYNYLQVAYFKINKLEKAVAAAHTFFVGNPEHMEMRQNLDYYQTMSGVKEADFRDLEAKPHM
  HEFRLGVRLYSEEKPQEAVPHLEAALQEYFVADEECRALCEGPYDYDGYNYLDYSADLFQAITDHYVQVL
  NCKQNCVTELASHPSREKPFEDFLPSHYNYLQFAYYNIGNYTQAIECAKTYLLFFPNDEVMHQNLAYYTA
  MLGEEEASSISPRENAEEYRRRSLLEKELLFFAYDIFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVR
  ISQEIGNLMKEIETLVEEKTKESLDVSRLTREGGPLLYEGISLTMNSKVLNGSQRVVMDGVISDDECQEL
  QRLTNAAATSGDGYRGQTSPHTPNEKFYGVTVLKALKLGQEGKVPLQSARMYYNVTEKVRRVMESYFRLD
  TPLYFSYSHLVCRTAIEESQAERKDSSHPVHVDNCILNAEALMCIKEPPAYTFRDYSAILYLNGDFDGGN
  FYFTELDAKTVTAEVQPQCGRAVGFSSGTENPHGVKAVTRGQRCAIALWFTLDPRHSERDRVQADDLVKM
  LFSPEEVDLPQEQPLPGQQGSPEPGEESLSDRGSLHKDEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 64.6 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001035874
• Locus ID: 56401
• UniProt ID: Q3V1T4
• Cytogenetics: 4 D2.1
• Refseq Size: 2844
• Refseq ORF: 2220
• Synonyms: 2410024C15Rik; Gros1; Lepre1
• Summary: Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts (By similarity).[UniProtKB/Swiss-Prot Function]