Mannan Binding Lectin (MBL2) (NM_000242) Human Recombinant Protein

CAT#: TP310143

Recombinant protein of human mannose-binding lectin (protein C) 2, soluble (opsonic defect) (MBL2), 20 µg

Product Images

Coomassie blue staining of purified MBL2 protein (Cat# TP310143). The protein was produced from HEK293T cells transfected with MBL2 cDNA clone (Cat# RC210143) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC210143 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLFPSLPLLLLSMVAASYSETVTCEDAQKTCPAVIACSSPGINGFPGKDGRDGTKGEKGEPGQGLRGLQ
  GPPGKLGPPGNPGPSGSPGPKGQKGDPGKSPDGDSSLAASERKALQTEMARIKKWLTFSLGKQVGNKFFL
  TNGEIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGITDEKTEGQFVDLTGNRLTYTNWNE
  GEPNNAGSDEDCVLLLKNGQWNDVPCSTSHLAVCEFPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 24 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_000233
• Locus ID: 4153
• UniProt ID: P11226
• Cytogenetics: 10q21.1
• Refseq Size: 3569
• Refseq ORF: 744
• Synonyms: COLEC1; HSMBPC; MBL; MBL2D; MBP; MBP-C; MBP1; MBPD
• Summary: This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes and binds to mannose and N-acetylglucosamine on many microorganisms, including bacteria, yeast, and viruses including influenza virus, HIV and SARS-CoV. This binding activates the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jun 2020]
• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades