dnaK / Hsp70 (508-638) Escherichia coli Protein

CAT#: SA6027

dnaK / Hsp70 (508-638) e. coli protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Escherichia coli
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIE SALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDV VDAEFEEVKD KK
• Predicted MW: 14.6 kDa
• Concentration: lot specific
• Purity: >95% by SDS-PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 10% Glycerol
• Preparation: Liquid purified protein
• Protein Description: Dnak (residues 508-638) of the substrate binding domain is α-helical and appears to act as a lid covering the substrate binding cleft. DnaK (amino acid 508-638) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N-terminus.
• Storage: Store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: DnaK, originally identified for its DNA replication by bacteriophage λ in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins.