Protein G Streptococcus Protein
Specifications
| Product Data | |
| Species | Streptococcus |
|---|---|
| Concentration | 1.0 mg/mL by UV absorbance at 280 nm |
| Purity | Protein G Alkaline Phosphatase Conjugated was prepared from chromatographically pure recombinant Protein G. Protein G Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein G. No reaction was observed against anti-Protein A. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Preparation | Liquid (sterile filtered) Ig fraction. |
| Recommended Dilution | ELISA,Immunohistochemistry,Western Blot |
| Protein Description | Alkaline Phosphatase Conjugated Protein G. |
| Note | Protein G Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader. |
| Storage | Store Protein G at 4° C before opening. DO NOT FREEZE. Protein G Alkaline Phosphatase Conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| UniProt ID | P19909 |
| Synonyms | ProG, Streptococcus G protein, ALP, Alk Phos |
| Summary | Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. While native Protein G binds to albumin, recombinant Protein G is designed to contain only immunoglobulin binding domains to ensure the maximum specific IgG binding capacity. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substrate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments. Protein G Alkaline Phosphatase Conjugated is ideal for investigators involved in Signal Transduction, Molecular Biology, Cancer, and Cell Signaling. |
| Protein Families | 000 of the stock concentration is suggested for this product., 500 to 1:19, Suitable for Immunoblotting (western or dot blot) |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
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