Protein G Streptococcus Protein

CAT#: RA101AP

Protein G streptococcus protein, 0.5 mg

Specifications

Product Data

• Species: Streptococcus
• Concentration: lot specific
• Purity: : This product was prepared from chromatographically pure Protein G.
• Conjugation: AP
• Buffer: State: Liquid (sterile filtered) Ig fraction.
  Buffer System: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140, 000 daltons).
  Presentation Label: AP
• Preparation: Liquid (sterile filtered) Ig fraction.
• Applications: Suitable for Immunoblotting (western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,500 to 1:19,000 of the stock concentration is suggested for this product.
• Protein Description: Alkaline Phosphatase Conjugated Protein G.
• Note: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein G.
  No reaction was observed against anti-Protein A.
• Storage: Store vial at 2-8°C before opening.
  DO NOT FREEZE!
  This product is stable at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
  Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
  Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.