Protein A (Peroxidase conjugated) Staphylococcus aureus Protein

CAT#: RA100HRP

Protein A (Peroxidase conjugated) s. aureus protein, 2 mg

Specifications

Product Data

• Species: Staphylococcus aureus
• Concentration: lot specific
• Purity: This product was prepared from chromatographically pure Protein A.
• Conjugation: HRP
• Buffer: State: Lyophilized purified protein
  Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Presentation Label: HRP
• Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
• Preparation: Lyophilized purified protein
• Applications: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/20,000-1/200,000.
  Western Blot: 1/10,000-1/40,000.
  Immunohistochemistry: 1/1,000-1/5,000.
  Note: Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).
• Protein Description: Horseradish Peroxidase (HRP) conjugated Protein A.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A.
  No reaction was observed against anti-Protein G
• Note: Molecular Weight: (protein A): 40,000 daltons.
  
  Caution: Do Not Add Sodium Azide.
• Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
  Dilute only prior to immediate use.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies.
  Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).