Protein A (AP conjugated) Staphylococcus aureus Protein
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Specifications
Product Data | |
Species | Staphylococcus aureus |
Concentration | lot specific |
Purity | This product was prepared from chromatographically pure Protein A. |
Conjugation | AP |
Buffer | State: Liquid (sterile filtered) Ig fraction. Buffer System: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml BSA (IgG and Protease free) as Stabilizer and 0.01% (w/v) Sodium Azide as Preservative. Label: Alkaline Phosphatase (Calf Intestine, Molecular Weight 140, 000 daltons). Presentation Label: AP |
Preparation | Liquid (sterile filtered) Ig fraction. |
Applications | Suitable for Western Blot (1:500- 1:2,500), ELISA and Immunohistochemistry (1:200- 1:1,000) as well as other phosphatase-avidin/biotin based enzymatic assays. Recommended dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the stock concentration is suggested for this product. |
Protein Description | Alkaline Phosphatase Conjugated Protein A. |
Note | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A. No reaction was observed against anti-Protein G. |
Storage | Store vial at 2-8°C before opening. DO NOT FREEZE!! This product is stable at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Stability | Shelf life: one year from despatch. |
Reference Data |
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