Angiopoietin-2 Human Protein

CAT#: DA3501

Angiopoietin-2 human recombinant protein, 5 µg

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Predicted MW: 60-70 kDa
• Purity: >95% pure by SDS-PAGE and visualised by silver stain
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized protein
  Buffer System: 10 mM Sodium Phosphate, pH 8.0
  Stabilizer: None
• Bioactivity: Biological: Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2μg/ml.
• Endotoxin: < 0.1 ng per µg of Ang-2
• Reconstitution: Restore in water to a concentration of 0.1-1.0 mg/ml.
  Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation: Lyophilized protein
• Protein Description: Recombinant Human ANG-2 is a C-terminal Histidine tagged glycoprotein which migrates with an apparent molecular mass of 60 – 70 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001112359
• Locus ID: 285
• UniProt ID: O15123
• Cytogenetics: 8p23.1
• Synonyms: AGPT2; ANG2; LMPHM10
• Summary: This gene belongs to the angiopoietin family of growth factors. The protein encoded by this gene is an antagonist of angiopoietin 1, and both angiopoietin 1 and angiopoietin 2 are ligands for the endothelial TEK receptor tyrosine kinase. Angiopoietin 2 is upregulated in multiple inflammatory diseases and is implicated in the direct control of inflammation-related signaling pathways. The encoded protein affects angiogenesis during embryogenesis and tumorigenesis, disrupts the vascular remodeling ability of angiopoietin 1, and may induce endothelial cell apoptosis. This gene serves a prognostic biomarker for acute respiratory distress syndrome. [provided by RefSeq, Aug 2020]
• Protein Families: Druggable Genome, Secreted Protein