Angiopoietin-2 Human Protein
|Predicted MW||60-70 kDa|
|Purity||>95% pure by SDS-PAGE and visualised by silver stain|
|Buffer||Presentation State: Purified
State: Lyophilized protein
Buffer System: 10 mM Sodium Phosphate, pH 8.0
|Bioactivity||Biological: Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2μg/ml.|
|Endotoxin||< 0.1 ng per µg of Ang-2|
|Reconstitution||Restore in water to a concentration of 0.1-1.0 mg/ml.
Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
|Protein Description||Recombinant Human ANG-2 is a C-terminal Histidine tagged glycoprotein which migrates with an apparent molecular mass of 60 – 70 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein.|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||AGPT2; ANG2; LMPHM10|
|Summary||This gene belongs to the angiopoietin family of growth factors. The protein encoded by this gene is an antagonist of angiopoietin 1, and both angiopoietin 1 and angiopoietin 2 are ligands for the endothelial TEK receptor tyrosine kinase. Angiopoietin 2 is upregulated in multiple inflammatory diseases and is implicated in the direct control of inflammation-related signaling pathways. The encoded protein affects angiogenesis during embryogenesis and tumorigenesis, disrupts the vascular remodeling ability of angiopoietin 1, and may induce endothelial cell apoptosis. This gene serves a prognostic biomarker for acute respiratory distress syndrome. [provided by RefSeq, Aug 2020]|
|Protein Families||Druggable Genome, Secreted Protein|
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