Beta-galactosidase tag Escherichia coli Protein

CAT#: BA254

Beta-galactosidase tag e. coli protein, 5 mg

Specifications

Product Data

• Species: Escherichia coli
• Purity: 600U/mg
• Buffer: State: Lyophilized purified protein from Escherichia coli
  Buffer System: PBS
  Stabilizer: 74% Sucrose
• Bioactivity: Specific: > 600 U/mg protein.
  One unit hydrolyses 1 μmole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC.
• Reconstitution: Restore with sterile distilled water or a dilute buffer of choice.
  Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
• Preparation: Lyophilized purified protein from Escherichia coli
• Applications: Functional Assays.
• Protein Description: Purified Beta-Galactosidase from E.coli.
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the protein undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Escherichia coli Beta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.