Tyrosine-protein kinase JAK2 (1014-1132, His-tag) Human Protein

CAT#: AR51307PU-S

Tyrosine-protein kinase JAK2 (1014-1132, His-tag) human protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMGE SPIFWYAPES LTESKFSVAS DVWSFGVVLY ELFTYIEKSK SPPAEFMRMI GNDKQGQMIV FHLIELLKNN GRLPRPDGCP DEIYMIMTEC WNNNVNQRPS FRDLALRVDQ IRDNMAG
• Tag: His-tag
• Predicted MW: 18.1 kDa
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
• Preparation: Liquid purified protein
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001309123
• Locus ID: 3717
• UniProt ID: O60674
• Cytogenetics: 9p24.1
• Synonyms: JTK10
• Summary: This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway