Galectin-4 (1-326, His-tag) Mouse Protein

CAT#: AR50608PU-N

Galectin-4 (1-326, His-tag) mouse recombinant protein, 0.25 mg

Product Images

Specifications

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MGSMAYVPAP GYQPTYNPTL PYKRPIPGGL SVGMSVYIQG MAKENMRRFH VNFAVGQDDG ADVAFHFNPR FDGWDKVVFN TMQSGQWGKE EKKKSMPFQK GKHFELVFMV MPEHYKVVVN GNSFYEYGHR LPVQMVTHLQ VDGDLELQSI NFLGGQPAAA PYPGAMTIPA YPAGSPGYNP PQMNTLPVMT GPPVFNPRVP YVGALQGGLT VRRTIIIKGY VLPTARNFVI NFKVGSSGDI ALHLNPRIGD SVVRNSFMNG SWGAEERKVA YNPFGPGQFF DLSIRCGMDR FKVFANGQHL FDFSHRFQAF QMVDTLEING DITLSYVQI
• Tag: His-tag
• Predicted MW: 38.8 kDa
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1 mM DTT
• Bioactivity: Biological:
  The ED50 for this effect is 3.9–7.8 ug/ml, measured by its ability to agglutinate human red blood cells.
  (Biological assay see "Protocols").
• Preparation: Liquid purified protein
• Applications: Protocol: Biological assay:
  1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g, Sodium citric acid 0.8g, Citr ic acid 0.055 g, D - glucose 2.05g in DW100 ml)
  2. Centrifuge at 15000rpm for 10 minutes and wash four times with PBS.
  3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
  4. Incubate for 1h at 37°C and wash four time s with PBS.
  6 . Load 50 ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red cell - in - PBS in u shaped wells.
  7 . Add 25 ul of serial diluted galectin protein in PBS to each well plate. (Round bot tom 96 well plate).
  8 . Incubate for 30min at room temperature to observe visible agglutination.
• Protein Description: Recombinant mouse Lgals4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_034836
• Locus ID: 16855
• UniProt ID: Q8K419, Q3UL35
• Cytogenetics: 7 16.94 cM
• Synonyms: gal-4
• Summary: Galectin that binds lactose and a related range of sugars.[UniProtKB/Swiss-Prot Function]