Isocitrate dehydrogenase / IDH (1-414, His-tag)

CAT#: AR50109PU-N

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Recombinant human IDH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.


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USD 820.00


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2 Weeks

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    • 500 ug


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Product Data
Description Recombinant human IDH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Species Human
Expression Host E. coli
Tag His-tag
Predicted MW 48.8 kDa
Concentration 1.0 mg/ml (determined by Bradford assay)
Purity > 95 % by SDS - PAGE
Buffer 20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT,0.1mM PMSF
Bioactivity > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C.
Preparation Liquid purified protein
Reference Data
RefSeq NP_001269315
Locus ID 3417
Cytogenetics 2q34
Synonyms HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
Summary Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
Protein Pathways Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

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68 Mouse Clones