Dickkopf-3 (DKK3) Human Protein

CAT#: AR31139PU-S

Dickkopf-3 (DKK3) human recombinant protein, 2 µg

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  APAPTATSAP VKPGPALSYP QEEATLNEMF REVEELMEDT QHKLRSAVEE MEAEEAAAKA SSEVNLANLP PSYHNETNTD TKVGNNTIHV HREIHKITNN QTGQMVFSET VITSVGDEEG RRSHECIIDE DCGPSMYCQF ASFQYTCQPC RGQRMLCTRD SECCGDQLCV WGHCTKMATR GSNGTICDNQ RDCQPGLCCA FQRGLLFPVC TPLPVEGELC HDPASRLLDL ITWELEPDGA LDRCPCASGL LCQPHSHSLV YVCKPTFVGS RDQDGEILLP REVPDEYEVG SFMEEVRQEL EDLERSLTEE MALREPAAAA AALLGGEEI
• Predicted MW: 36.3 kDa
• Purity: >98% pure by SDS-PAGE and HPLC analyses
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized (0.2μ Sterile filtered) purified protein
• Bioactivity: Biological: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 E1 cells. The expected ED₅₀ for this effect is 2.0–4.0 ng/ml.
• Preparation: Lyophilized (0.2μ Sterile filtered) purified protein
• Protein Description: Recombinant human DKK-3 expressed in CHO cells is a glycoprotein that has a calculated molecular weight of 36.3 kDa and contains 329 amino acid residues.
  Due to glycosylation, human DKK-3 migrates at an apparent molecular weight of approximately 39-49 kDa by SDS-PAGE analysis under non-reducing conditions.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001018067
• Locus ID: 27122
• UniProt ID: Q9UBP4
• Cytogenetics: 11p15.3
• Synonyms: REIC; RIG
• Summary: This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Secreted Protein