G-CSF Rat Protein

CAT#: AR31104PU-S

G-CSF rat recombinant protein, 2 µg

Specifications

Product Data

• Species: Rat
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MKKIPLLTVS SLPPSLPLPR SFLLKSLEQV RKIQARNTEL LEQLCATYKL CHPEELVLFG HSLGIPKASL SSCSSQALQQ TKCLSQLHSG LFLYQGLLQA LAGISSELAP TLDMLHLDVD NFATTIWQQM ESLGVAPTVQ PTQSTMPIFT SAFQRRAGGV LVTSYLQSFL ETAHHALHHL PRPAQKHFPE SLFISI
• Predicted MW: 21.6 kDa
• Purity: >98% pure by SDS-PAGE gel and HPLC analyses
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized (0.2µ Sterile filtered) purified protein
  Buffer System: 10 mM Sodium Citrate, pH 4.0
• Bioactivity: Biological: Determined by its ability to stimulate the proliferation of Mouse NFS-60 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.
  Specific: ≥ 2 x 10⁷ units/mg.
• Endotoxin: < 0.1 ng/μg of protein (< 1EU/μg)
• Reconstitution: Restore in water to a concentration of 0.1-1.0 mg/ml.
  Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation: Lyophilized (0.2µ Sterile filtered) purified protein
• Protein Description: Recombinant Rat G-CSF is a 21.6 kDa protein consisting of 196 amino acid residues.
• Note: Centrifuge the vial prior to opening.
• Storage: Prior to reconstitution store at 2-8°C for 6 months, at RT for one month
  or at -20°C to -80°C for longer.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_058800
• Locus ID: 25610
• Cytogenetics: 10q31
• Synonyms: Gcsf
• Summary: putative hematopoetic growth factor for neutrophils; used as a treatment for cyclic hematopoesis in humans and dogs [RGD, Feb 2006]