Bleomycin hydrolase (1-455, His-tag) Human Protein

CAT#: AR09703PU-N

Bleomycin hydrolase (1-455, His-tag) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MSSSGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQRAQ HVFQHAVPQE GKPITNQKSS GRCWIFSCLN VMRLPFMKKL NIEEFEFSQS YLFFWDKVER CYFFLSAFVD TAQRKEPEDG RLVQFLLMNP ANDGGQWDML VNIVEKYGVI PKKCFPESYT TEATRRMNDI LNHKMREFCI RLRNLVHSGA TKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM GALAE
• Tag: His-tag
• Predicted MW: 54.7 kDa
• Concentration: lot specific
• Purity: >90%
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol
• Bioactivity: Specific: > 1,000 pmole/min/ug.
  Measured by the hydrolysis of Met-AMC at pH 7.5, at 37°C.
• Preparation: Liquid purified protein
• Applications: Protocol: Activity assay
  1. Prepare 100 ul of recombinant BLMH protein with various concentrations (0.06 ug, 0.03 ug) in assay buffer and equilibrate to 37°C for 30 minutes.
  (Assay buffer: 50 mM Tris-HCl, 5 mM EDTA, 10 mM DTT, pH 7.5)
  2. Add 50 ul of 2mM Met-AMC.
  3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
  - Met-AMC (Enzo, Cat.No, BML-P236-0025)
  - 96 Well Polystyrene Microplate, black (greiner bio-one, Cat.No, 655077)
  - Fluorescent plate reader (PerkinElmer, VICTOR X3)
• Protein Description: Recombinant human BLMH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_000377
• Locus ID: 642
• UniProt ID: Q13867
• Cytogenetics: 17q11.2
• Synonyms: BH; BMH
• Summary: Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Protease