Galectin-8 (1-317, His-tag) Human Protein

CAT#: AR09626PU-N

Galectin-8 (1-317, His-tag) human recombinant protein, 50 µg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA DVAFHFNPRF KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST QASSLELTEI SRENVPKSGT PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV RNSFLQESWG EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI DTLEINGDIH LLEVRSW
• Tag: His-tag
• Predicted MW: 37.9 kDa
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl, 1 mM DTT, 10% glycerol
• Bioactivity: Biological:
  The ED50 for this effect is is 5-10 ug/ml, measured by its ability to agglutinate human red blood cells.
  (Biological assay see "Protocols").
• Preparation: Liquid purified protein
• Applications: Protocol: Biological Assay :
  1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g, Sodium citric aci d 0.8g, Citric acid 0.055 g, D - glucose 2.05g in DW100 ml)
  2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
  3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
  4. Incubate for 1h at 37°C and wash four times with PBS.
  5 . Dilute packed cells in PBS to give 4% red cell suspension.
  6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
  7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
  8 . Incubate for 30min at room temperature to observe visible agglutination.
• Protein Description: Recombinant human LGALS8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_006490
• Locus ID: 3964
• UniProt ID: O00214
• Cytogenetics: 1q43
• Synonyms: Gal-8; PCTA-1; PCTA1; Po66-CBP
• Summary: This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]