Peroxiredoxin-4 / PRDX4 (38-271, His-tag) Human Protein

CAT#: AR09413PU-L

Peroxiredoxin-4 / PRDX4 (38-271, His-tag) human recombinant protein, 0.5 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN
• Tag: His-tag
• Predicted MW: 28.8 kDa
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
• Bioactivity: Specific: Approximately 230-310 pmole/min/µg.
  Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX4 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25°C for 1 minute.
• Preparation: Liquid purified protein
• Applications: Protocol: Activity Assay:
  1. Prepare a 50ul reaction mix into a suitable container :
  The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol.
  2. Add 5 ul of recombinant PRDX4 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer.
  3. Incubate at 25°C for 2 minutes.
  4. Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min.
  5. Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction.
  6. Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN)
  7. Record the increase in A475nm.
• Protein Description: Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_006397
• Locus ID: 10549
• UniProt ID: Q13162, V9HW63
• Cytogenetics: Xp22.11
• Synonyms: AOE37-2; AOE372; HEL-S-97n; PRX-4
• Summary: The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome