Carbonic anhydrase 2 (1-260) Human Protein

CAT#: AR09206PU-N

Carbonic anhydrase 2 (1-260) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK
• Predicted MW: 29.2 kDa
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 50 mM NaCl, 10% glycerol
• Bioactivity: Biological: Specific activity is 50-70 nmoles/min/ug and was obtained by measuring the increase in the amount of p-nitrophenol by its esterase activity.
  Specific activity is defined as the amount of p-nitrophenol that 1ug of enzyme can reduce at 25°C for 1 minute.
  Activity Assay
  1. Prepare assay buffer into a suitable container: The final concentrations are 12.5 mM Tris, 75 mM NaCl, pH 7.5.
  2. Dilute 4-Nitrophenyl-Acetate(4-NPA) to 2 mM in Assay Buffer.
  3. Dilute recombinant CA2 protein with various concentrations (1ug, 2ug, 5ug) in 50 ul assay buffer.
  
  4. Load 50 μL of 2mM 4-NPA into diluted recombinant CA2 solutions, and start the reaction by adding 50 μL of 2 mM Substrate.
  5. Read at a wavelength of 405 nm (bottom read) in kinetic mode for 5 minutes.
• Preparation: Liquid purified protein
• Protein Description: Recombinant human CA2 was expressed in E.coli and purified by using conventional chromatography.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_000058
• Locus ID: 760
• UniProt ID: P00918, V9HW21
• Cytogenetics: 8q21.2
• Synonyms: CA-II; CAC; CAII; Car2; HEL-76; HEL-S-282
• Summary: The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism