Interleukin-3 / IL3 (20-152, His-tag) Human Protein

CAT#: AR09049PU-N

Interleukin-3 / IL3 (20-152, His-tag) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MAPMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
• Tag: His-tag
• Predicted MW: 17.3 kDa
• Concentration: lot specific
• Purity: >90% pure by SDS PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris pH 8.0, 0.2 mM PMSF, 10% glycerol
• Bioactivity: Biological: The ED50 for this effect is <0.53 ng/ml . Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
  Activity Assay
  Cell line: TF1 (human erythroleukemic cell).
  Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF.
  Assay medium - 10% FBS RPMI 1640 without Gm-CSF.
  Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul).
  Starvation: 24hr, Assay medium.
  Incubation time: 24 hr (after sample treatment).
  Concentration range: 0.048 ng/ml - 25 ng/ml.
  Detection method: MTT assay.
• Preparation: Liquid purified protein
• Protein Description: Recombinant human IL-3, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.
• Storage: Store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_000579
• Locus ID: 3562
• UniProt ID: P08700
• Cytogenetics: 5q31.1
• Synonyms: IL-3; MCGF; MULTI-CSF
• Summary: The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway