IFNG / Interferon gamma Human Protein
IFNG / Interferon gamma human recombinant protein, 0.1 mg
|Description||IFNG / Interferon gamma human recombinant protein, 0.1 mg|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ|
|Predicted MW||16.9 kDa|
|Purity||>95% pure by SDS-PAGE|
|Buffer||Presentation State: Purified
State: Liquid purified protein
Buffer System: PBS, pH 7.4 containing 10% Glycerol
|Bioactivity||Biological: Measured in a cytotoxicity assay using WiDr cells. The ED50 for this effect is ≤ 5 ng/ml.|
|Endotoxin||< 1.0 EU per 1 µg of Protein (determined by LAL method).|
|Preparation||Liquid purified protein|
|Protein Description||Recombinant Human interferon-γ was expressed in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Additional amino acid (Methionine) is attached at N-terminus.|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Summary||This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]|
|Protein Families||Druggable Genome, Secreted Protein|
|Protein Pathways||Allograft rejection, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Proteasome, Regulation of autophagy, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Type I diabetes mellitus|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.