B Raf (BRAF) Mutant (V600E) ICC Control Section

CAT#: TS600155P5

Transient overexpression of B Raf (BRAF) Mutant (V600E) in mammalian cells, paraffin embedded, 5 µm sections

Product Images

Immunohistology staining on BRAF mutant (V600E) overexpressed cytosection TS600155P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC400155, 5 micron sections, 40x magnification.

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  Immunohistology staining on Negative Control overexpressed cytosection TC400001 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. 5 micron sections, 40x magnification.

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  Immunohistology staining on BRAF mutant (V600E) overexpressed cytosection TS600155P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC400155, 5 micron sections, 40x magnification

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  Immunohistology staining on BRAF mutant (V600E) overexpressed cytosection TS600155P5 with rabbit anti BRAF (V600E) clone OTIR1F3 C/N TA592461 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC400155, 5 micron sections, 40x magnification

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  Immunohistology staining on BRAF overexpressed cytosection TS411013P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC211013, 5 micron sections, 40x magnification

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  Immunohistology staining on BRAF overexpressed cytosection TS411013P5 with rabbit anti BRAF (V600E) clone OTIR1F3 C/N TA592461 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC400155, 5 micron sections, 40x magnification

• Immunohistology staining on Negative Control overexpressed cytosection TC400001 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. 5 micron sections, 40x magnification

• Immunohistology staining on Negative Control overexpressed cytosection TC400001 with rabbit anti BRAF (V600E) clone OTIR1F3 C/N TA592461 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. 5 micron sections, 40x magnification

• Immunohistology staining on BRAF mutant (V600E) overexpressed cytosection TS600155P5 with rabbit anti BRAF (V600E) clone OTIR1F3 C/N TA592461 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC400155, 5 micron sections, 40x magnification

• Immunohistology staining on BRAF overexpressed cytosection TS411013P5 with mouse anti BRAF clone OTI5A9 C/N TA500431 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC400155, 5 micron sections, 40x magnification

• Immunohistology staining on Negative Control overexpressed cytosection TC400001 with mouse anti BRAF clone OTI5A9 C/N TA500431 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. 5 micron sections, 40x magnification

• Immunohistology staining on Negative Control overexpressed cytosection TC400001 with rabbit anti BRAF (V600E) clone OTIR1F3 C/N TA592461 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. 5 micron sections, 40x magnification

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Specifications

Product Data

• Mutation Description: V600E
• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC400155
• Tag: C-Myc-DDK
• Detection Antibodies: DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags (TA592569)
• Target Detection Antibodies: B Raf (BRAF) Mutant (V600E) Rabbit Monoclonal Antibody [Clone ID: OTIR1F3] (TA592461)
• Accession Number: NM_004333
• Other Names: B-raf; B-RAF1; BRAF1; NS7; RAFB1
• Storage: Room Temperature
• Stability: Slides are guaranteed for a year from the date of receipt if proper storage instructions were followed.
• Preparation: HEK293T cells were transiently transfected with TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs. After harvesting, the cultured cells were fixed in formalin & dehydrated before embedding in paraffin. 5 um sections of the FFPE cell pellet blocks are cut and mounted on positively charged SuperFrost slides.
• Note: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Reference Data

• Locus ID: 673
• Cytogenetics: 7q34
• Protein Families: Druggable Genome, Protein Kinase