C9orf114 (SPOUT1) (NM_016390) Human Mass Spec Standard

CAT#: PH322092

C9orf114 MS Standard C13 and N15-labeled recombinant protein (NP_057474)

Product Images

Coomassie blue staining of purified SPOUT1 protein (Cat# TP322092). The protein was produced from HEK293T cells transfected with SPOUT1 cDNA clone (Cat# RC222092) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC222092
• Predicted MW: 42.1 kDa
• Protein Sequence:
  Sequence Link

  >RC222092 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAERGRKRPCGPGEHGQRIEWRKWKQQKKEEKKKWKDLKLMKKLERQRAQEEQAKRLEEEEAAAEKEDRG
  RPYTLSVALPGSILDNAQSPELRTYLAGQIARACAIFCVDEIVVFDEEGQDAKTVEGEFRGVGKKGQACV
  QLARILQYLECPQYLRKAFFPKHQDLQFAGLLNPLDSPHHMRQDEESEFREGIVVDRPTRPGHGSFVNCG
  MKKEVKIDKNLEPGLRVTVRLNQQQHPDCKTYHGKVVSSQDPRTKAGLYWGYTVRLASCLSAVFAEAPFQ
  DGYDLTIGTSERGSDVASAQLPNFRHALVVFGGLQGLEAGADADPNLEVAEPSVLFDLYVNTCPGQGSRT
  IRTEEAILISLAALQPGLIQAGARHT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057474
• RefSeq Size: 4302
• RefSeq ORF: 1128
• Synonyms: C9orf114; CENP-32; CENP32; HSPC109
• Locus ID: 51490
• UniProt ID: Q5T280
• Cytogenetics: 9q34.11
• Summary: Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase (PubMed:20813266, PubMed:25657325). Also involved in chromosome alignment (PubMed:20813266). May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis (PubMed:25657325). Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level (PubMed:28431233).[UniProtKB/Swiss-Prot Function]
• Protein Families: Druggable Genome