NHP2L1 (SNU13) (NM_001003796) Human Mass Spec Standard

CAT#: PH321735

NHP2L1 MS Standard C13 and N15-labeled recombinant protein (NP_001003796)

Product Images

Coomassie blue staining of purified SNU13 protein (Cat# TP321735). The protein was produced from HEK293T cells transfected with SNU13 cDNA clone (Cat# RC221735) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC221735
• Predicted MW: 14.2 kDa
• Protein Sequence:
  Sequence Link

  >RC221735 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTEADVNPKAYPLADAHLTKKLLDLVQQSCNYKQLRKGANEATKTLNRGISEFIVMAADAEPLEIILHLP
  LLCEDKNVPYVFVRSKQALGRACGVSRPVIACSVTIKEGSQLKQQIQSIQQSIERLLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001003796
• RefSeq Size: 1520
• RefSeq ORF: 384
• Synonyms: 15.5K; FA-1; FA1; NHP2L1; NHPX; OTK27; SNRNP15-5; SPAG12; SSFA1
• Locus ID: 4809
• UniProt ID: P55769, Q6FHM6
• Cytogenetics: 22q13.2
• Summary: Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
• Protein Pathways: Spliceosome