C1orf80 (AIDA) (NM_022831) Human Mass Spec Standard

CAT#: PH317521

AIDA MS Standard C13 and N15-labeled recombinant protein (NP_073742)

Product Images

Coomassie blue staining of purified AIDA protein (Cat# TP317521). The protein was produced from HEK293T cells transfected with AIDA cDNA clone (Cat# RC217521) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC217521
• Predicted MW: 35 kDa
• Protein Sequence:
  Sequence Link

  >RC217521 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSEVTRSLLQRWGASFRRGADFDSWGQLVEAIDEYQILARHLQKEAQAQHNNSEFTEEQKKTIGKIATCL
  ELRSAALQSTQSQEEFKLEDLKKLEPILKNILTYNKEFPFDVQPVPLRRILAPGEEENLEFEEDEEEGGA
  GAGSPDSFPARVPGTLLPRLPSEPGMTLLTIRIEKIGLKDAGQCIDPYITVSVKDLNGIDLTPVQDTPVA
  SRKEDTYVHFNVDIELQKHVEKLTKGAAIFFEFKHYKPKKRFTSTKCFAFMEMDEIKPGPIVIELYKKPT
  DFKRKKLQLLTKKPLYLHLHQTLHKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_073742
• RefSeq Size: 3020
• RefSeq ORF: 918
• Synonyms: C1orf80
• Locus ID: 64853
• UniProt ID: Q96BJ3
• Cytogenetics: 1q41
• Summary: Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling (By similarity).[UniProtKB/Swiss-Prot Function]