RNF93 (TRIM15) (NM_033229) Human Mass Spec Standard

CAT#: PH307716

TRIM15 MS Standard C13 and N15-labeled recombinant protein (NP_150232)

Product Images

Coomassie blue staining of purified TRIM15 protein (Cat# TP307716). The protein was produced from HEK293T cells transfected with TRIM15 cDNA clone (Cat# RC207716) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC207716
• Predicted MW: 52.1 kDa
• Protein Sequence:
  Sequence Link

  >RC207716 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPATPSLKVVHELPACTLCAGPLEDAVTIPCGHTFCRLCLPALSQMGAQSSGKILLCPLCQEEEQAETPM
  APVPLGPLGETYCEEHGEKIYFFCENDAEFLCVFCREGPTHQAHTVGFLDEAIQPYRDRLRSRLEALSTE
  RDEIEDVKCQEDQKLQVLLTQIESKKHQVETAFERLQQELEQQRCLLLARLRELEQQIWKERDEYITKVS
  EEVTRLGAQVKELEEKCQQPASELLQDVRVNQSRCEMKTFVSPEAISPDLVKKIRDFHRKILTLPEMMRM
  FSENLAHHLEIDSGVITLDPQTASRSLVLSEDRKSVRYTRQKKNLPDSPLRFDGLPAVLGFPGFSSGRHR
  WQVDLQLGDGGGCTVGVAGEGVRRKGEMGLSAEDGVWAVIISHQQCWASTSPGTDLPLSEIPRGVRVALD
  YEAGQVTLHNAQTQEPIFTFTASFSGKVFPFFAVWKKGSCLTLKG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_150232
• RefSeq Size: 2241
• RefSeq ORF: 1395
• Synonyms: RNF93; ZNF178; ZNFB7
• Locus ID: 89870
• UniProt ID: Q9C019, Q5SRL0
• Cytogenetics: 6p22.1
• Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Transcription Factors