LSM7 (NM_016199) Human Mass Spec Standard

CAT#: PH305835

LSM7 MS Standard C13 and N15-labeled recombinant protein (NP_057283)

Product Images

Coomassie blue staining of purified LSM7 protein (Cat# TP305835). The protein was produced from HEK293T cells transfected with LSM7 cDNA clone (Cat# RC205835) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC205835
• Predicted MW: 11.6 kDa
• Protein Sequence:
  Sequence Link

  >RC205835 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADKEKKKKESILDLSKYIDKTIRVKFQGGREASGILKGFDPLLNLVLDGTIEYMRDPDDQYKLTEDTRQ
  LGLVVCRGTSVVLICPQDGMEAIPNPFIQQQDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057283
• RefSeq Size: 536
• RefSeq ORF: 309
• Synonyms: YNL147W
• Locus ID: 51690
• UniProt ID: Q9UK45
• Cytogenetics: 19p13.3
• Summary: Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Apr 2004]
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation, Spliceosome