PDE6D (NM_002601) Human Mass Spec Standard

CAT#: PH303172

PDE6D MS Standard C13 and N15-labeled recombinant protein (NP_002592)

Product Images

Coomassie blue staining of purified PDE6D protein (Cat# TP303172). The protein was produced from HEK293T cells transfected with PDE6D cDNA clone (Cat# RC203172) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203172
• Predicted MW: 17.4 kDa
• Protein Sequence:
  Sequence Link

  >RC203172 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQ
  MEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLV
  STSRVRLFYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002592
• RefSeq Size: 1214
• RefSeq ORF: 450
• Synonyms: JBTS22; PDED
• Locus ID: 5147
• UniProt ID: O43924, Q6IB24
• Cytogenetics: 2q37.1
• Summary: This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism