CKS1 (CKS1B) (NM_001826) Human Mass Spec Standard

CAT#: PH302933

CKS1B MS Standard C13 and N15-labeled recombinant protein (NP_001817)

Product Images

Coomassie blue staining of purified CKS1B protein (Cat# TP302933). The protein was produced from HEK293T cells transfected with CKS1B cDNA clone (Cat# RC202933) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202933
• Predicted MW: 9.7 kDa
• Protein Sequence:
  Sequence Link

  >RC202933 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSHKQIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTHLMSESEWRNLGVQQSQGWVHYMIHEPEPHILLFR
  RPLPKKPKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001817
• RefSeq Size: 834
• RefSeq ORF: 237
• Synonyms: CKS1; ckshs1; PNAS-16; PNAS-18
• Locus ID: 1163
• UniProt ID: P61024, Q5T178
• Cytogenetics: 1q21.3
• Summary: CKS1B protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS1B mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects a specialized role for the encoded protein. At least two transcript variants have been identified for this gene, and it appears that only one of them encodes a protein. [provided by RefSeq, Sep 2008]
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Pathways in cancer, Small cell lung cancer