NME2 (NM_001018139) Human Mass Spec Standard

CAT#: PH300680

NME2 MS Standard C13 and N15-labeled recombinant protein (NP_001018149)

Product Images

Coomassie blue staining of purified NME2 protein (Cat# TP300680). The protein was produced from HEK293T cells transfected with NME2 cDNA clone (Cat# RC200680) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200680
• Predicted MW: 17.3 kDa
• Protein Sequence:
  Sequence Link

  >RC200680 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNS
  GPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELV
  DYKSCAHDWVYE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001018149
• RefSeq Size: 682
• RefSeq ORF: 456
• Synonyms: NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF
• Locus ID: 4831
• UniProt ID: P22392, Q6FHN3
• Cytogenetics: 17q21.33
• Summary: Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism