Adrenomedullin (ADM) (NM_001124) Human Mass Spec Standard

CAT#: PH300507

ADM MS Standard C13 and N15-labeled recombinant protein (NP_001115)

Product Images

Coomassie blue staining of purified ADM protein (Cat# TP300507). The protein was produced from HEK293T cells transfected with ADM cDNA clone (Cat# RC200507) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200507
• Predicted MW: 20.4 kDa
• Protein Sequence:
  Sequence Link

  >RC200507 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLVSVALMYLGSLAFLGADTARLDVASEFRKKWNKWALSRGKRELRMSSSYPTGLADVKAGPAQTLIRP
  QDMKGASRSPEDSSPDAARIRVKRYRQSMNNFQGLRSFGCRFGTCTVQKLAHQIYQFTDKDKDNVAPRSK
  ISPQGYGRRRRRSLPEAGPGRTLVSSKPQAHGAPAPPSGSAPHFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001115
• RefSeq Size: 1606
• RefSeq ORF: 555
• Synonyms: AM; PAMP
• Locus ID: 133
• UniProt ID: P35318
• Cytogenetics: 11p15.4
• Summary: The protein encoded by this gene is a preprohormone which is cleaved to form two biologically active peptides, adrenomedullin and proadrenomedullin N-terminal 20 peptide. Adrenomedullin is a 52 aa peptide with several functions, including vasodilation, regulation of hormone secretion, promotion of angiogenesis, and antimicrobial activity. The antimicrobial activity is antibacterial, as the peptide has been shown to kill E. coli and S. aureus at low concentration. [provided by RefSeq, Aug 2014]
• Protein Families: Druggable Genome, Secreted Protein