Rb (RB1) CytoSection

CAT#: TS406933P5

Transient overexpression of RB1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack

Product Images

Immunohistology staining on RB1 overexpressed cytosection TS406933P5 with mouse anti DDK clone OTI11C3 C/N TA180144 at 1:400 dilution 4C O/N. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC206933, 5 micron sections, 40x magnification

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: TrueORF Clone RC206933
• Tag: C-MYC/DDK
• Detection Antibodies: Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
• Target Detection Antibodies: Rb (RB1) Mouse Monoclonal Antibody [Clone ID: OTI3F11] (TA805656)
• Accession Number: NM_000321, NP_000312
• Other Names: OSRC; p105-Rb; p110-RB1; pp110; PPP1R130; pRb; RB
• Storage: Room Temperature
• Stability: Slides are guaranteed for a year from the date of receipt if proper storage instructions were followed.
• Preparation: HEK293T cells were transiently transfected with TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs. After harvesting, the cultured cells were fixed in formalin & dehydrated before embedding in paraffin. 5 µm sections of the FFPE cell pellet blocks are cut and mounted on positively charged SuperFrost slides.
• Note: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Reference Data

• RefSeq: NP_000312
• Locus ID: 5925
• Cytogenetics: 13q14.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer