Rat Icam1 ELISA KIT (96 T)

CAT#: EA800184

Rat sICAM-1 ELISA KIT

Datasheet
Size
    • 96 T

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Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 4 hours
Signal Colorimetric
Curve Range 31.25-2000pg/ml
Sample Type Serum, plasma, Cell culture supernatant
Sample Volume 100 uL
Specificity This assay recognizes both natural and recombinant rat sICAM-1
Sensitivity 15 pg/ml
Reactivities Rat
Interference No significant interference observed with available related molecules.
Components
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials
  • Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials
  • Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial
  • Standard /Sample Diluent - 16 ml/vial,1 bottle
  • Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle
  • Streptavidin-HRP Diluent - 16 ml/vial,1 bottle
  • Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle
  • Substrate Solution - 12 ml/vial,1 bottle
  • Stop Solution - 12 ml/vial,1 bottle
  • Plate Cover Seals,4 pieces
Background Rat Intercellular Adhesion Molecule 1 (ICAM-1), also known as CD54, is an 80-110 kDa, type I transmembrane glycoprotein that is expressed on a variety of cell types. The molecule is 545 amino acids (aa) in length and contains a 27 aa signal sequence, a 466 aa extracellular region, a 25 aa transmembrane segment, and a 27 aa cytoplasmic domain. The extracellular region contains five Ig-like domains and eleven potential N-linked glycosylation sites. The first N-terminal Ig domain (D1) binds LFA-1 (lymphocyte function associated antigen type 1), while the third domain (D3) binds Mac-1, also known as integrin alpha-M. The cytoplasmic domain, while short, can both transduce intracellular signals (via MAP kinase) and interact with the cytoskeleton. While membrane ICAM-1 exists as a dimer, monomeric ICAM-1 is competent to bind LFA-1 as well. Soluble forms of ICAM-1 are generated via proteolytic processing, reportedly by MMP-9 and elastase. Soluble, dimeric ICAM-1 circulates and binds LFA-1 with high avidity.
Gene Symbol Icam1
Gene ID 25464

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