Chicken TGFB1 ELISA KIT (96 T)

CAT#: EA800010

Chicken TGF-β1 Immunoassay

Product Images

Representative standard curve for TGF-β1 ELISA

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 4 hours
• Signal: Colorimetric
• Curve Range: 31.25-2000pg/ml
• Sample Type: Serum, plasma, Cell culture supernatant
• Sample Volume: 100 uL
• Specificity: This assay recognizes both natural and recombinant Chicken TGF-β1
• Sensitivity: 6 pg/mL
• Reactivities: Chicken
• Interference: No significant interference observed with available related molecules.
• Components:
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials
  • lyophilized Biotin-Conjugated antibody,2 vials
  • Concentrated Streptavidin-HRP,1 vial
  • Standard /Sample Diluent,1 bottle
  • Biotin-Conjugate antibody Diluent,1 bottle
  • Streptavidin-HRP Diluent,1 bottle
  • 20 x Wash Buffer Concentrate,1 bottle
  • Substrate Solution,1 bottle
  • Stop Solution ,1 bottle
  • Plate Cover Seals,4 pieces
  • 1N HCl,1 bottle
  • 1N NaOH,1 bottle
• Background: TGF-β1 (transforming growth factor beta 1) was first identified in human platelets as a protein with a molecular mass of 25 kilodaltons with a potential role in wound healing. TGF- β1, TGF- β2, and TGF- β3 all function through the same receptor signaling systems. They are members of the large TGF-β superfamily. TGF-β proteins are highly pleiotropic cytokines that regulate processes such as immune function, proliferation and epithelial mesenchymal transition. It was later characterized as a large protein precursor (containing 390 amino acids) that was proteolytically processed to produce a mature peptide of 112 amino acids .TGF-β activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins. Although different isoforms of TGFβ are naturally associated with their own distinct LAPs, the TGF-β1 LAP is capable of complexing with, and inactivating, all other Chicken TGF-β isoforms and those of most other species. Mutations within the LAP are associated with Camurati Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-β1.
• Gene Symbol: TGFB1
• Gene ID: 100873157