Canine IFNG ELISA KIT (96 T)

CAT#: EA800007

Canine IFN-γ Immunoassay

Product Images

Representative standard curve for IFN-γ ELISA

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 4 hours
• Signal: Colorimetric
• Curve Range: 18.75-1200pg/ml
• Sample Type: Serum, plasma, Cell culture supernatant
• Sample Volume: 100 uL
• Specificity: This assay recognizes both natural and recombinant Canine IFN-?
• Sensitivity: 10 pg/mL.
• Reactivities: Canine
• Interference: No significant interference observed with available related molecules.
• Components:
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,1200pg/ml upon reconstitution,2 vials
  • lyophilized, Biotin-Conjugated antibody,2 vials
  • Concentrated Streptavidin-HRP,1 vial
  • Standard /sample Diluent,1 bottle
  • Biotin-Conjugate antibody Diluent,1 bottle
  • Streptavidin-HRP Diluent,1 bottle
  • 20 x Wash Buffer Concentrate,1 bottle
  • Substrate Solution,1 bottle
  • Stop Solution,1 bottle
  • Plate Cover Seals,4 pieces
• Background: IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons . It plays key roles in both the innate and adaptive immune response against viral and intracellular bacterial infections and for tumor control . It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. IFN-γ activates the cytotoxic activity of innate immune cells such as macrophages and NK cells . IFN-γ production by NK cells and antigen-presenting cells (APCs) promotes the cell mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increased class I and class II MHC expression, and enhancing peptide antigen presentation . The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2 and Stat1 . The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis .
• Gene Symbol: IFNG
• Gene ID: 403801