Rat Angpt2 ELISA KIT (1 x 96 wells)

CAT#: EA102409

For quantitative detection of rat Angiopoietin-2 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for rat Angiopoietin-2
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10000pg/ml
• Specificity: This kit is used for quantitative detection of rat Angiopoietin-2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Sensitivity: <15pg/ml
• Reactivities: Rat
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- rat Angiopoietin-2 antibody | 1
  • Lyophilized recombinant rat Angiopoietin-2 standard | 10ng/tube×2
  • Biotinylated anti- rat Angiopoietin-2 antibody | 100μl (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 100μl (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Wash Buffer (25x) | 20ml
  • Plate Sealers | 4 pieces
• Background: Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
• Gene Symbol: Angpt2
• Gene ID: 89805