Human IGF1 ELISA KIT (1 x 96 wells)

CAT#: EA102183

For quantitative detection of human IGF-1 in cell culture supernates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human IGF-1
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10000pg/ml
• Specificity: This kit is used for quantitative detection of Human IGF-1
• Sensitivity: <50pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with IGF-2.
• Components:
  • 96-well plate precoated with anti-human IGF1 antibody | 1
  • Lyophilized recombinant human IGF1 standard | 10 ng/tube x 2
  • Biotinylated anti-human IGF1 antibody | 100 ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12 ml
  • ABC diluent buffer | 12 ml
  • TMB color developing agent | 10 ml
  • TMB stop solution | 10 ml
  • Adhesive cover | 4
  • Wash Buffer (25x) | 20 ml
• Background: Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. It is mapped to 12q23.2. The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
• Gene Symbol: IGF1
• Gene ID: 3479