Human TNF alpha ELISpot Kit, 5 x 96-well (pre-coated plates)

CAT#: EA101486

Human TNF alpha ELISpot Kit, 5 x 96-well (pre-coated plates)

Specifications

Product Data

• Format: 5 x 96-well (pre-coated plates)
• Assay Type: Enzyme-Linked ImmunoSpot (ELISpot)
• Assay Length: 3h after cell stimulation
• Signal: Colorimetric
• Specificity: Recognizes natural human TNF-a
• Cross Reactivity: No cross reactivity with other human cytokines. Cross reacivity with macac TNF-a. No interference with TNFR I and TNFR II
• Components: Pre-coated ELISpot kits include precoated PVDF plates, Detection antibody, Alkaline phosphatase conjugate, BSA, BCIP/NBT ready-to-use substrate buffer.
• Background: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
• Gene Symbol: TNF
• Gene ID: 7124