Human sVEGFR1/sFLT1 ELISA Kit

CAT#: EA100379

Human sVEGFR1/sFLT1 ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human sVEGFR1/sFLT1
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10,000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human sVEGFR1
• Sensitivity: <30pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti-human FLT1 antibody | 1
  • Lyophilized recombinant human FLT1 standard | 10ng/tube x 2
  • Biotinylated anti-human FLT1 antibody | 100ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml
• Background: sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.
• Gene Symbol: FLT1
• Gene ID: 2321